Mass separation and in vitro immunological activity of membrane-fractionated polysaccharides from fruiting body and mycelium of Agaricus subrufescens

Autor: Carlos R. Zanetti, Margarida Matos de Mendonça, Aguinaldo R. Pinto, Márcio José Rossi, Carla Maísa Camelini, Douglas Bardini Silveira, José Carlos Cunha Petrus, Álvaro José Celmer
Rok vydání: 2012
Předmět:
Zdroj: Biotechnology and Bioprocess Engineering. 17:804-811
ISSN: 1976-3816
1226-8372
DOI: 10.1007/s12257-011-0645-y
Popis: Membrane technology has been applied to separate polysaccharides from Agaricus subrufescens (ASPs). The membrane-retained fractions and unfractionated preparations have been tested for in vitro immunological activity. Both the microfiltration (MF) and ultrafiltration (UF1) membranes were able to separate high-molecular weight polysaccharides from fruiting body (ASP-FB) and submerge-fermented mycelium (ASP-SmF) extracts. All fractions showed immunostimulatory effects on RAW 264.7 macrophages, measured by TNF-α, iNOs gene expression, and NO production. In contrast, antibody and proliferation levels in B lymphoblastoid SKW 6.4 cells were significantly increased after treatment with ASP-FB, but did not with ASP-SmF preparations. The ASPs- and LPS-induced stimulation could be differentiated by the finding that polymyxin B, a specific inhibitor of LPS, did not significantly affect the immunoactivating response and proliferation activity of ASPs on macrophages and B cells, respectively. Furthermore, the ASP-FB treatment was unable to induce IL-6 production by B cells unlike LPS activation, sustaining distinct signaling pathways for ASP-FB and LPS. The overall results provided additional information about the action of ASPs on the immune system and support the membrane method to separate and concentrate high- molecular weight ASPs for immunopharmacological and biotechnological applications.
Databáze: OpenAIRE
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