| Autor: |
Л.a. ЛяпиN, a.M. yльяN, Б.a. КyдpяMoB, A.M. Ulianov, B.A. Kudrjashov, L.A. Liapina |
| Rok vydání: |
1973 |
| Předmět: |
|
| Zdroj: |
Thrombosis Research. 3:589-603 |
| ISSN: |
0049-3848 |
| DOI: |
10.1016/0049-3848(73)90119-9 |
| Popis: |
It has been found that heparin (Hp) forms a complex with fibrin stabilizing factor (FSF) in vitro. The formation of complex FSF-Hp has been testified by electrophoresis, analytical ultracentrifugation, spectral analysis in the regions of ultra-violet and ultra-red absorption and by study of differential spectrum of complex FSF-Hp. Maximum of specific biochemical activity of the complex FSF-Hp was estabilished when the weight relation between Hp and FSF reached 1:4, at pH 7,3. Complex FSF-Hp diminished specific activity when its formation was carried out in the conditions of raising or reducing weight ratio of Hp. The activity totally disappeared when ratio Hp:FSF was reached 1:1 or 1:15. Complex FSF-Hp obtained in optimal conditions acted as an inhibitor of polymerization (aggregation) of fibrin monomer. It also carried out the lysis of non-stabilized fibrin in the presence or absence of E-aminocaproic acid. The mentioned biochemical activity of complex FSF-Hp is very similar to one of complex fibrinogen-heparin, formation of which in vitro and in vivo and physiological significance we investigated before (2,3) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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