Gene expression and in vitro replication of bovine gammaherpesvirus type 4

Autor: E. Louge-Uriarte, Andrea Elizabeth Verna, S.G. Delgado, Anselmo Carlos Odeón, E. Gonzalez-Altamiranda, Susana Beatriz Pereyra, Pedro Edgardo Morán, F. Romeo, Sandra Perez
Rok vydání: 2021
Předmět:
Zdroj: Archives of Virology. 166:535-544
ISSN: 1432-8798
0304-8608
DOI: 10.1007/s00705-020-04898-8
Popis: In vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions remain to be answered about the role of BoHV-4 in endometrial cells. The aim of this study was to compare the replication and gene expression of BoHV-4 in cell lines and bovine reproductive tract primary cells as an in vitro model for the study of this virus. We demonstrated that BoHV-4 strains differ in their in vitro growth kinetics and gene expression but have the same cell type preference. Our results demonstrate that BoHV-4 replicates preferentially in bovine endometrial cells (BEC). However, its replication capacity extends to various cell types, since all cells that were tested were permissive to BoHV-4 infection. The highest virus titers were obtained in BEC cells. Nevertheless, virus replication efficiency could not be fully predicted from the mRNA expression profiles. This implies that there are multiple cell-type-dependent factors and strain properties that determine the level of BoHV-4 replication. The results of this study provide relevant information about the in vitro behavior of two field isolates of BoHV-4 in different cell cultures. These findings may be useful for the design of future in vitro experiments to obtain reliable results not only about the pathogenic role of BoHV-4 in the bovine female reproductive tract but also in the development of efficient antiviral strategies.
Databáze: OpenAIRE
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