RNase H-catalyzed site-specific deadenylylation of rabbit alpha- and beta- globin mRNAs. Secondary structure of 3'-noncoding regions

Autor:	Calvin P.H. Vary, J. N. Vournakis
Rok vydání:	1984
Předmět:	chemistry.chemical_classification Messenger RNA Sequence analysis Cell Biology Biology Biochemistry Molecular biology Enzyme chemistry biology.protein Polyadenylate Ribonuclease Globin RNase H Molecular Biology Protein secondary structure
Zdroj:	Journal of Biological Chemistry. 259:3299-3307
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(17)43294-7
Popis:	A general method is described for the removal of 3'-terminal polyadenylate tracts from eukaryotic messenger RNA to generate essentially homogeneous length products that can be 3'-end labeled and sequenced. Hybridization of specific oligodeoxyribonucleotides was used to direct ribonuclease H to the junction of the 3'-noncoding region and the polyadenylate sequence of rabbit alpha and beta globin mRNAs. Site-specific deadenylylation of both globin mRNAs is demonstrated by partial enzymatic sequence analysis following 3'-terminal labeling with 5'-pCp (where p indicates the labeled phosphate group). The secondary structure of the 3'-noncoding region is studied by enzymatic digestion with S1 nuclease and cobra venom ribonuclease. Structural features of the 3'-noncoding regions of these mRNAs are described, including the protein synthesis termination and the poly(A) addition recognition sites.
Databáze:	OpenAIRE
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