Biodiesel Potential of Oleaginous Yeast Biomass by NMR Spectroscopic Techniques

Autor:	Valnei S. Cunha, A. S. Sarpal, Julio J. Amaral, Wanderley de Souza, Marianne M. Monnerat, Paulo R. M. Silva, Juliana Lopes Martins, Romeu J. Daroda
Rok vydání:	2014
Předmět:	chemistry.chemical_classification Biodiesel Chromatography General Chemical Engineering Glyceride Extraction (chemistry) Energy Engineering and Power Technology Fatty acid Solvent chemistry.chemical_compound Fuel Technology chemistry Proton NMR Organic chemistry Gas chromatography Methanol
Zdroj:	Energy & Fuels. 28:3766-3777
ISSN:	1520-5029 0887-0624
DOI:	10.1021/ef402516x
Popis:	Analytical strategies based on NMR (1H and 13C), IR (infrared), and GC (gas chromatography) techniques have been developed for the molecular level characterization of Soxhlet and ultrasonic solvent extracts of yeast biomass samples generated on a lab scale by different yeast, feed, and diverse culture conditions, with an objective to explore biodiesel potential. The extraction efficiency of each solvent (cyclohexane, chloroform, methanol) toward extraction of neutral lipids (total glycerides (TG), free fatty acids (FFA), and polar lipids have been determined and compared with regards to the nature of fatty acid components extracted in each solvent fractions. The fatty acid composition of yeast extracts has been found to be similar to vegetable oils, mostly rich in C16:0, 18:0, and C18:N (N = 1–3) fatty acids as indicated by the combined NMR, GC, and GC-MS analyses. The analytical protocol developed has established that 1H NMR techniques can be used directly and rapidly without any sample treatment and pri...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7b749b27502714cf4055dd2102b607aa https://doi.org/10.1021/ef402516x Zobrazit plný text záznamu