Monodispersed spherical shaped selenium nanoparticles (SeNPs) synthesized byBacillus subtilisand its toxicity evaluation in zebrafish embryos
| Autor: | Krishnan Sundar, Subburaman Chandramohan, Azhaguchamy Muthukumaran |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Absorption (pharmacology) Polymers and Plastics biology Scanning electron microscope Metals and Alloys Energy-dispersive X-ray spectroscopy chemistry.chemical_element Nanoparticle 02 engineering and technology Bacillus subtilis 021001 nanoscience & nanotechnology biology.organism_classification Surfaces Coatings and Films Electronic Optical and Magnetic Materials Biomaterials 03 medical and health sciences 030104 developmental biology chemistry Zeta potential Fourier transform infrared spectroscopy 0210 nano-technology Selenium Nuclear chemistry |
| Zdroj: | Materials Research Express. 5:025020 |
| ISSN: | 2053-1591 |
| DOI: | 10.1088/2053-1591/aaabeb |
| Popis: | Selenium is one of the essential elements involved in antioxidative and antiinflammatory effects in human body. By naturally, selenium ions are metabolised and converted into nano selenium. Now a days there is an increasing attention on applications of nanoparticles in therapeutic field. In the present study Bacillus subtilis was used to convert sodium selenite to SeNPs. The synthesized SeNPs were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM) coupled with Energy Dispersive X ray spectroscopy (EDX). The presence of SeNPs was confirmed by the formation of red colour. The bands were sharp with broad absorption peaks at 3562 cm−1 and 1678−1 cm in FTIR which showed that the bacterial proteins were responsible for the reduction of sodium selenite to SeNPs. The average size of the SeNPs was 334 nm and were spherical in shape with uniform distribution. The XRD data confirmed that SeNPs were of amorphous in nature. The zeta potential of SeNPs was negative in charge which indicated high stability. In the present study zebrafish embryos were used to study the toxicity of SeNPs and the results showed that the concentration beyond 10 μg ml−1 leads to toxic effects in embryos/hatchlings. The lesser concentration of SeNPs can be useful in various biomedical applications. |
| Databáze: | OpenAIRE |
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