Integrative Proteomic Analysis of Digestive Tract Glycosidases from the Invasive Golden Apple Snail, Pomacea canaliculata
| Autor: | Michelle L. Colgrave, Federico A. Dellagnola, Israel A. Vega, Omar Mendoza-Porras, Sophia Escobar-Correas |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology biology Extracellular digestion General Chemistry Snail biology.organism_classification Biochemistry Freshwater snail Transcriptome 03 medical and health sciences 030104 developmental biology biology.animal biology.protein Glycoside hydrolase Amylase Digestion Pomacea canaliculata |
| Zdroj: | Journal of Proteome Research. 18:3342-3352 |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | The freshwater snail Pomacea canaliculata, an invasive species of global significance, possesses a well-developed digestive system and diverse feeding mechanisms enabling the intake of a wide variety of food. The identification of glycosidases in adult snails would increase the understanding of their digestive physiology and potentially generate new opportunities to eradicate and/or control this invasive species. In this study, liquid chromatography coupled to tandem mass spectrometry was applied to define the occurrence, diversity, and origin of glycoside hydrolases along the digestive tract of P. canaliculata. A range of cellulases, hemicellulases, amylases, maltases, fucosidases, and galactosidases were identified across the digestive tract. The digestive gland and the contents of the crop and style sac yield a higher diversity of glycosidase-derived peptides. Subsequently, peptides derived from 81 glycosidases (46 proteins from the public database and 35 uniquely from the transcriptome database) that were distributed among 13 glycoside hydrolase families were selected and quantified using multiple reaction monitoring mass spectrometry. This study showed a high glycosidase abundance and diversity in the gut contents of P. canaliculata which participate in extracellular digestion of complex dietary carbohydrates. Salivary and digestive glands were the main tissues involved in their synthesis and secretion. |
| Databáze: | OpenAIRE |
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