Popis: |
(1) In vitro and in vivo studies on the midgut, haemolymph, fat body and Malpighian tubules fo Aeshna cyanea nymphs using [1- 14 C]palmityl alcohol with and without unlabelled oleic acid revealed that free fatty alcohol was readily absorbed from the midgut lumen. (2) The absorbed fatty alcohol was partly assimilated in the midgut epithelium to fatty acid and esterified predominantly to polar lipids and acyl glycerols. (3) The non-assimilated alcohol was esterified with endogenous or dietary fatty acid to wax ester, which was released into the haemolymph and transported to the lipocytes of the fat body and Malpighian tubules. (4) Fatty alcohol assimilation was highest in the midgut wall and fat body, while wax ester synthesis was highest in the midgut lumen, followed by the midgut epithelium and fat body. (5) Dietary oleic acid reduced the concentration of free fatty alcohol as a result of pre-absorptive wax ester synthesis in the midgut lumen and decreased the rate of fatty alcohol absorption. |