Self-assembled scorpion venom proteins cross-linked chitosan nanoparticles for use in the immunotherapy
| Autor: | Alessandra Daniele-Silva, Arnóbio Antônio da Silva-Júnior, A.L.P.F. Caroni, Matheus F. Fernandes-Pedrosa, Fiamma Gláucia-Silva, Alice R. Oliveira, Karla Samara Rocha Soares |
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| Rok vydání: | 2017 |
| Předmět: |
Tityus serrulatus
Nanoparticle 02 engineering and technology 010402 general chemistry 01 natural sciences Chitosan chemistry.chemical_compound Dynamic light scattering Materials Chemistry Zeta potential Physical and Theoretical Chemistry Bovine serum albumin Spectroscopy Gel electrophoresis Chromatography biology Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification Atomic and Molecular Physics and Optics 0104 chemical sciences Electronic Optical and Magnetic Materials Biophysics biology.protein Nanocarriers 0210 nano-technology |
| Zdroj: | Journal of Molecular Liquids. 241:540-548 |
| ISSN: | 0167-7322 |
| DOI: | 10.1016/j.molliq.2017.06.071 |
| Popis: | Immunotherapy is the specific treatment used against lethality caused by the scorpions. New immunoadjuvants reduce side effects and the toxicity of antigens compared with conventional formulations. The aim of this study was to establish how the composition and procedure parameters affect the formation and structural properties of protein-loaded chitosan nanoparticles for potential use in the immunotherapy against the scorpion envenomation. The bovine serum albumin (BSA) and the venom from scorpion Tityus serrulatus were entrapped in the nanoparticles produced by low energy ionic gelation method. The protein loading ranged from 5% to 30% w/w. The protein interactions with nanoparticles were monitored using zeta potential, dynamic light scattering, scanning electronic microscopy, Fourier transform infrared spectroscopy, and gel electrophoresis to assure sub-200 nm particles. The proteins were successfully entrapped in the small and narrow-sized chitosan nanoparticles. The slow protein release was diffusion-controlled dependent on protein/copolymer ratio in the nanoparticles. A scaffold of a low energy ionic gelation method for producing small-sized, spherical, stable, and cationic biodegradable and biocompatible nanoparticles was demonstrated as promising nanocarriers for proteins or negatively charged molecules from the biotechnology source. The nanoparticles successfully modulated the slow release of BSA and the proteins from the scorpion Tityus serrulatus. |
| Databáze: | OpenAIRE |
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