Study of microwave induced secondary structural evolution in Antheraea assamensis (muga) silk nanoparticles

Autor:	I. Banerjee, Prithvi C. Asapur, Santhosh Kumar Mahapatra
Rok vydání:	2020
Předmět:	Circular dichroism SILK biology Chemical engineering Chemistry Nanoparticle Fibroin Antheraea assamensis Fourier transform infrared spectroscopy biology.organism_classification Protein secondary structure Random coil
DOI:	10.22541/au.159818464.44415652
Popis:	Silk Nanoparticles were synthesized using conventional desolvation and microwave-assisted radiolysis method from Muga silk fibroin. The secondary structure evolution due to two different synthesis methods was investigated using FTIR and Circular Dichroism (CD) spectroscopy. The microwave synthesized nanoparticles showed enhanced β-sheet content than desolvation synthesized nanoparticles. The conformational changes of Muga silk fibroin protein to nanoparticle followed an ordered transition from random coil to α-helix then from α-helix to β-turn and from β-turn to β-sheets where α-helix and β-turn are the intermediate forms before getting stabilized to the metastable β-sheets structure. The thermodynamics involved in secondary structure evolution was studied from CD analysis and activation energy involved in the formation of the secondary structure was determined.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7af12a5dd58f1f88341e6f07118b4336 https://doi.org/10.22541/au.159818464.44415652 Zobrazit plný text záznamu