Seminal plasma hormonal profile of Arabian stallions that are classified ‘good’ or ‘poor’ for semen freezing

Autor: Gamal A. El Sisy, Diya A. El-Badry, Amal M. Abo El-Maaty
Rok vydání: 2016
Předmět:
Zdroj: Asian Pacific Journal of Reproduction. 5:453-458
ISSN: 2305-0500
DOI: 10.1016/j.apjr.2016.10.012
Popis: Objective To relate seminal plasma reproductive and metabolic hormone profile to both semen freezability and post-thaw semen characteristics. Methods Semen was collected from each Arabian stallion (N = 16) for five consecutive weeks and evaluated before conducting this study. Seminal plasma was collected during semen processing for measuring leptin, insulin, Insulin like growth factor-I (IGF-1), cortisol, testosterone, estradiol and thyroid hormones. Stallions were classified into good (N = 10) and poor freezer (N = 6). Semen post-thaw motility (%), viability index, membrane and acrosome integrity (%) were also evaluated. Results Good freezer stallions had significantly (P = 0.0001) high cortisol, estradiol, insulin, IGF-1, T3 and T4, but significantly low leptin (P = 0.003). Post-thaw sperm viability index (P = 0.0001), membrane integrity (%) (P = 0.002) and motility (%) (P = 0.0001) at 0, 1, 2 and 3 h of good freezer stallions was significantly high compared to poor freezers. Testosterone, insulin and IGF-1 in seminal plasma of good freezer stallions had significant (P
Databáze: OpenAIRE