Heterologous production of Rhizopus oryzae lipase in Pichia pastoris using the alcohol oxidase and formaldehyde dehydrogenase promoters in batch and fed-batch cultures
| Autor: | Francisco Valero, David Resina, Pau Ferrer, José Luis Montesinos, Oriol Cos |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Environmental Engineering biology Biomedical Engineering Triacylglycerol lipase Rhizopus oryzae Heterologous Bioengineering Promoter biology.organism_classification Pichia pastoris Alcohol oxidase Biochemistry biology.protein Lipase Formaldehyde dehydrogenase Biotechnology |
| Zdroj: | Biochemical Engineering Journal. 26:86-94 |
| ISSN: | 1369-703X |
| DOI: | 10.1016/j.bej.2005.04.005 |
| Popis: | A Rhizopus oryzae lipase (ROL) has been expressed in Pichia pastoris as a reporter gene using two different regulated promoters. Both phenotypes, Muts and Mut+, have been used for the expression under the control of alcohol oxidase 1 promoter (PAOX1). Moreover, the new formaldehyde dehydrogenase 1 promoter (PFLD1) has been tested. PFLD1 allows the design of methanol-free culture strategies, being methylamine a less volatile and flammable inducer. The comparison in batch and fed-batch cultures of the two promoters has shown that Muts (PAOX1) is somewhat more efficient than PFLD1 and Mut+ (PAOX1) systems, with a 1.1- and 1.4-fold higher specific productivity (U lipase/g biomass h), respectively. However, the productivity in all three systems was very similar (2500 U lipase/l h). These results demonstrate that the expression of heterologous proteins under the transcriptional control of PFLD1 is a promising alternative to the well-known PAOX1. |
| Databáze: | OpenAIRE |
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