Developmental potential of in vitro produced bovine embryos following cryopreservation and single-embryo transfer

Autor: J.M.C. Reinders, W.F. Rall, Th.A.M. Kruip, Y.A. Wurth
Rok vydání: 1994
Předmět:
Zdroj: Theriogenology. 42:1275-1284
ISSN: 0093-691X
DOI: 10.1016/0093-691x(94)90247-g
Popis: Information on the developmental potential of in vitro-produced, cryopreserved bovine embryos following single-embryo transfer to synchronized recipients is limited. The aim of this study was to compare the pregnancy rates of vitrified, conventionally cryopreserved, and control (unfrozen) in vitro embryos. The embryos were cryopreserved either by Vitrification in Solution 3a (VS3a) or by conventional slow freezing in 10% glycerol. Forty-two percent (n=121) of the recipients established pregnancy when in vitro produced morulae to expanded blastocyst stage embryos were transferred immediately following culture. Singnificantly more morulae on Day 7 and 8 of culture established pregnancy ( 11 14 ) than blastocysts on Days 7 to 9 of culture ( 40 107 ; P 12 35 ) than those on Days 7 and 8 of culture ( 39 86 ; P
Databáze: OpenAIRE