On-column electrochemical redox derivatization for enhancement of separation selectivity of liquid chromatography

Autor:	Yumiko Noda, Fumiko Yabiku, Kazunori Saitoh, Kohta Koichi, Masami Shibukawa, Marc D. Porter
Rok vydání:	2008
Předmět:	Catechol Working electrode Chromatography Hydroquinone Elution Organic Chemistry General Medicine Electrochemistry Biochemistry Redox High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Derivatization
Zdroj:	Journal of Chromatography A. 1180:66-72
ISSN:	0021-9673
DOI:	10.1016/j.chroma.2007.12.003
Popis:	An on-column electrochemical redox derivatization for enhancement of high-performance liquid chromatography (HPLC) separation selectivity is presented using electrochemically modulated liquid chromatography (EMLC) and porous graphitic carbon (PGC) as the packing material. PGC therefore serves two purposes: it acts both as a chromatographic stationary phase and as a working electrode. The capability of on-column electrochemical redox derivatization was evaluated using hydroquinone and catechol as model compounds. By manipulation of the applied potential, hydroquinone and catechol will migrate as equilibrium mixtures, hydroquinone and p-benzoquinone and catechol and o-benzoquinone in the potential region of 25-125 mV and 150-200 mV (vs. Ag/AgCl), respectively. These redox reactions can be used as secondary chemical equilibria so that the corresponding equilibrium mixtures elute as single peaks and their retention times can be controlled by alterations in the potential applied to the PGC stationary phase. Homogeneity of the redox activity of the PGC stationary phase applied potential was also demonstrated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7ac76ea5baadd8d1cabcdbd8dbfb08b8 https://doi.org/10.1016/j.chroma.2007.12.003 Zobrazit plný text záznamu Full Text from ScienceDirect