Paris saponin I induces G2/M cell cycle arrest and apoptosis in human gastric carcinoma SGC7901 cells

Autor:	Rong-Rong Zhou, Ze-Bing Huang, Guan-Sheng Hu, Bao-Xin Zhang, Mei-Fang Xiao, Xue-Gong Fan, Xia-Hong Dai, Xinchun He
Rok vydání:	2011
Předmět:	Cyclin-dependent kinase 1 Cell cycle checkpoint Cell growth Biomedical Engineering Cell cycle Biology Biochemistry Molecular biology Cell biology Biomaterials chemistry.chemical_compound chemistry Apoptosis Genetics Propidium iodide Fragmentation (cell biology) Cyclin B1 Earth-Surface Processes
Zdroj:	Journal of Huazhong University of Science and Technology [Medical Sciences]. 31:768-772
ISSN:	1993-1352 1672-0733
DOI:	10.1007/s11596-011-0674-y
Popis:	The aim of this study was to investigate the effect of Paris saponin I (PS I) on human gastric carcinoma cell growth and apoptosis and to explore the potential mechanisms. The proliferation of SGC7901 cells was monitored by the MTT cell viability assay, while the nuclear morphology of apoptotic cells was assessed by Hoechst 33258 staining. Flow cytometry was performed to analyze the cell cycle progression of propidium iodide (PI)-stained SGC7901 cells and the apoptotic rate of annexin V/PI-stained cells. Western blotting was used to examine the expression of several cell cycle proteins, including cyclin B1 and Cdk1, and the apoptosis-regulated proteins Bcl-2, Bax, cytochrome c, procaspase-9, and procaspase-3. The MTT assay demonstrated that PS I could induce significant dose- and time-dependent inhibition of SGC7901 cell proliferation. Marked morphological changes, including condensation of chromatin, nuclear fragmentation and apoptotic bodies were clearly shown on Hoechst 33258 staining. PSI treatment also resulted in the disruption of the cell cycle at G2/M and the induction of apoptosis. Following PSI treatment, the cell cycle-related proteins cyclin B1 and Cdk1 were down-regulated. Expression of the pro-apoptotic protein Bax was increased, while anti-apoptotic protein Bcl-2 decreased. PSI treatment resulted in elevated cytoplasmic cytochrome c and activation of the apoptotic proteases caspase-9 and caspase-3. These data indicate that PS acts as an inhibitor of proli I feration in SGC7901 cells by inducing cell cycle arrest and mitochondria-dependent apoptosis. PSI is a potential therapeutic agent against human gastric carcinoma.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7ac4bb816155a08a2ddfdefe4c2ac063 https://doi.org/10.1007/s11596-011-0674-y Zobrazit plný text záznamu