Induction of α-Amylase in Barley Endosperm by Substrate Levels of Glutamate and Aspartate
| Autor: | Alan G. Galsky, James A. Lippincott |
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| Rok vydání: | 1971 |
| Předmět: | |
| Zdroj: | Plant Physiology. 47:551-554 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.47.4.551 |
| Popis: | Incubation of embryoless barley (Hordeum vulgare) half-seeds for 24 hours with 0.1 m glutamate or aspartate resulted in the release of 17 to 48% as much alpha-amylase as did incubation with 260 mmum gibberellin. With incubation periods of 48 to 51 hours these amino acids were on the average about half as active as response-saturating concentrations of gibberellin, and in some experiments they were essentially as active. Citric acid cycle intermediates, glycolytic pathway intermediates, and cofactors of these pathways failed to induce alpha-amylase synthesis, while the following compounds were active: asparagine, homoserine, diaminopimelate, isoleucine, methionine, glutamine, ornithine, citrulline, argininosuccinate, and delta-aminolevulinate. However, threonine, lysine, beta-alanine, alanine, gamma-aminobutyrate, alpha-ketobutyrate, proline, arginine, glycine, leucine, and putrescine were inactive. Two patterns were noted in the list of active and inactive compounds: (a) all of the active compounds contain an amino group and are biosynthetically derived from citric acid cycle intermediates; and (b) biosynthetic precursors of the amino acids arginine, proline, threonine, and lysine were active whereas these amino acids were not. |
| Databáze: | OpenAIRE |
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