HLA class I expression and chromosomal deletions at 6p and 15q in head and neck squamous cell carcinomas
| Autor: | J. van Kuik, R.A. de Weger, S. Wiertsema, Manita Feenstra, M. Veltkamp, P.J. Slootweg, M. G. J. Tilanus, J. G. Van Den Tweel |
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| Rok vydání: | 1999 |
| Předmět: |
Pathology
medicine.medical_specialty Immunology Chromosome General Medicine Human leukocyte antigen Biology Biochemistry Loss of heterozygosity stomatognathic diseases medicine.anatomical_structure Chromosomal region Genetics medicine Cancer research biology.protein Immunology and Allergy Immunohistochemistry TAP2 TAP1 Lymph node |
| Zdroj: | Tissue Antigens. 54:235-245 |
| ISSN: | 0001-2815 |
| DOI: | 10.1034/j.1399-0039.1999.540304.x |
| Popis: | Loss at the chromosomal region 6p21.3 is a frequent event in head and neck squamous cell carcinomas (HNSCC). Since the human leukocyte antigen (HLA) complex is located at 6p21.3, loss of heterozygosity (LOH) of this region may provide tumour cells with an immune-escape tumour phenotype. In the present study, we have studied the correlation of HLA class I, TAP1 and TAP2 expression and LOH at 6p21.3. HLA class I and TAP1 and TAP2 protein expression was analysed by immunohistochemical procedures. A panel of 41 HNSCC with downregulated HLA class I expression was selected for LOH studies using 5 microsatellite markers located at 6p21.3 (D6S105, D6S265, D6S276, D6S273, D6S291) and 2 markers located at the chromosome 6 centromere (D6S473) and the 6p telomere (D6S277). In addition, LOH of the beta-2-nmicroglobulin (beta2m) gene was studied using 2 microsatellite markers flanking the beta2m gene (D15S126 and D15S153) and was correlated with beta2m and HLA class I expression. In 20/41 (49%) of the HNSCC, allelic loss for at least one locus at 6p21.3 was found. Loss at 15q was found in 4/10 (40%) HNSCC with downregulated beta2m expression and in 12/41 (29%) HNSCC with downregulated HLA class I expression. Our data show that downregulation of HLA class I expression is correlated with loss of chromosomal regions at 6p21.3 in HNSCC. In addition, LOH at 6p21.3 and 15q in 10 paired samples of DNA derived from the primary HNSCC, the lymph node metastases and from peripheral blood lymphocytes (PBLs) was studied. Five (5/10) primary tumours contained the same deletion as the corresponding lymph node metastases. The other cases contained deletions either in the primary tumour (3 cases) or in the lymph node metastases (1 case) or no deletions at all (1 case). |
| Databáze: | OpenAIRE |
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