Clinical and Histological Features of Intraoral Flap and a Preliminary Study of DNA Methylation of Mucosalization

Autor: Yixing Li, Feixin Liang, Tao Yu, Chuangui Zeng, Wan Fan, Zhuoqian Zhou, Xueru Chen, Qinchao Tang
Rok vydání: 2020
Předmět:
Zdroj: Indian Journal of Surgery. 82:559-565
ISSN: 0973-9793
0972-2068
Popis: Flaps have been routinely used to reconstruct intraoral defects. Flaps after reconstruction tend to develop, with time, a mucosa-like appearance during a process called “mucosalization.” Many studies have described histological changes of mucosalization, but only a few of them mention the associated clinical factors. Our aim was to determine the possible predictive factors related to mucosalization of flaps employed for oral cavity reconstruction, and to verify whether skin inflammation-specific DNA methylation occurs in mucosalized flaps. In this study, 140 patients underwent reconstruction of the oral cavity defects with flaps, of which 39 showed “mucosalization” changes. Histological changes of “mucosalization” are characterized by inflammatory infiltration, accompanied by a reduction of thickness of the horny layer and elimination of skin appendages. These changes are affected by clinical differences with sex and type and site of flaps. We also found that DNA methyltransferase 1 was highly expressed in the epithelium of mucosalized flaps, and less in unmucosalized flaps, suggesting their involvement in the inflammation-dependent DNA methylation of mucosalization. To summarize, our results demonstrated that most intraorally placed flaps maintain skin features, except in the event of heavy inflammation. Mucosalization varies in association with sex of the subject and type and site of flaps. DNA methyltransferase 1 might correlate with mucosalization, suggesting that DNA methylation may be involved in chronic inflammation in mucosalization.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje