| Autor: |
Edward D. Gomperts, Parvin Izadi |
| Rok vydání: |
1979 |
| Předmět: |
|
| Zdroj: |
Thrombosis and Haemostasis. |
| ISSN: |
2567-689X |
| DOI: |
10.1055/s-0039-1687305 |
| Popis: |
AT III was assessed in different batches of two commercial Factor IX concentrates. The assay procedures were the Laurell QIE for AT IIIAGN with functional activity being assayed using both a commercial kit and the chromogenic substrate S2160. There was considerable variation between the separate products and among the different batches. In one product. AT IIIAGM varied between 2.6 and 15%, the other between 75 and 145% of pooled control plasma. Functional activity assessed with the commercial kit varied between batch and products but generally was greater than antigen in one product; less than antigen in the other. S2160 dependent assays failed to corre Hate with these levels. The chromogenic system demonstrated considerably more functional activity in all samples tested. Heparin failed to induce heparin co-factor (HCF} activity as assessed by the S2160-IIa, S2222-FXa and thrombin-fibrinogen systems. This observation was confirmed by 2DIE where all samples failed to show increased heparin-induced migration. It can be assumed that AT III in at least some commercial concentrates contains AT IIIAGN. This AT III has no HCF activity but functional activity varies considerably depending upon method of assay. α1 AT and α2M antigen are present, but in considerably smaller guantities than AT III. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
