Popis: |
Although liquid column chromatography (LC) had been used as a means of chemical separation for many years prior to 1969, it was not accepted as a method useful for rapid, routine analysis, due to the relatively long times required to achieve resolution. Significant theoretical and technological advances have since been made in this field, and excellent separations can now be achieved within a few minutes using high-efficiency LC column packings. Liquid chromatography offers many advantages over gas-liquid chromatography (GLC) in that no restrictions are placed on the size, volatility, or thermal stability of the sample molecules. In addition, LC offers tremendous flexibility in the choice of mobile and stationary phases such that most sample components can be conveniently resolved using some appropriate combination of mobile and stationary phases. |