Kinetics of isotope incorporation into the desoxyribonucleic acid (DNA) of tissues: Life span and generation time of cells

Autor: Demetrios A. Rigas
Rok vydání: 1958
Předmět:
Zdroj: The Bulletin of Mathematical Biophysics. 20:33-70
ISSN: 1522-9602
0007-4985
DOI: 10.1007/bf02476560
Popis: The dynamics of cell multiplication and differentiation in tissues in asteady state and the kinetics of isotope incorporation into the DNA have been theoretically analyzed. Equations have been derived, with the aid of which thegeneration time, thelife span, and the distribution or rate of death of the cells can be obtained if the tissue is in asteady state, i.e., if the number of cells is maintained constant by constant, equal rates of cell division and cell death and if the mean DNA content per cell is also constant. An equation has also been derived which gives thegeneration time in the case of logarithmic multiplication of cells. Two special cases have been analyzed: InCase 1, the isotope is considered as being introduced into the metabolic system at zero time only; inCase 2, the specific activity of the DNA precursor is considered as being maintained constant. The use of the method has been illustrated by an example in which thegeneration time and themean, themedian, and themode life span, as well as the curve of the rate of death of leukocytes in a patient with chronic leukemic granulocytic leukemia, have been obtained from the rate of P32 incorporation into the DNA. The merits and the limitations of the method are discussed.
Databáze: OpenAIRE
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