IMPULSE ACTIVITY DIFFERENTIALLY REGULATES LEUENKEPHALIN AND CATECHOLAMINE (CA) CHARACTERS IN THE ADRENAL MEDULLA
| Autor: | Joshua E. Adler, E F La Gamma, Ira B. Black |
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| Rok vydání: | 1984 |
| Předmět: | |
| Zdroj: | Pediatric Research. 18:140A-140A |
| ISSN: | 1530-0447 0031-3998 |
| Popis: | Trans-synaptic impulse activity has long been known to increase CA biosynthesis and CA synthetic enzymes in the adrenal medulla. Since opiate peptides are co-stored and co-released with CA's in medullary cells, and since medullary opiate peptides may be important modulators of the sympathoadrenal stress response, we examined trans-synaptic regulation of the putative peptide neurohumour, leucine-enkephalin (ENK) and the CA enzymes, tyrosine hydroxylase (TH) and phenyl-ethanolamine-N-methyl transferase (PNMT) in the rat adrenal medulla in vivo and in vitro. Surgical denervation of the adrenal or pharmacologic blockade of synaptic transmission, treatments known to decrease CA traits, increased ENK-like immunoreactivity to 150% of control. Medullae explanted to culture exhibited a 50 fold rise in ENK in 4 days, whereas TH remained constant, and PNMT decreased to a new baseline. Depolarizing concentrations of K+ prevented the accumulation of ENK while not affecting TH or PNMT. Our studies suggest that enkephalinergic and catecholamine characters are differentially regulated by impulse activity and depolarization in the adrenal medulla. Independent regulation of adrenal neuropeptides and CA enzymes may allow new therapeutic approaches to shock, hypoxemia, or stress-induced analgesia through alteration of specific neurohumoral mechanisms. (Supported by NIH Grants HL00756, NS10259, and HD12108). |
| Databáze: | OpenAIRE |
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