Impact of CdSe/ZnS quantum dots on the development of zebrafish embryos
| Autor: | Zhe Zhang, Fengjiao Deng, Zhike He, Yi Liu, Wansu Xu, Yong Lei, Shan Huang, Qi Xiao |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
animal structures
Materials science Bioengineering In situ hybridization In vivo Notochord otorhinolaryngologic diseases medicine General Materials Science Chronic toxicity Zebrafish Microinjection biology technology industry and agriculture Embryo General Chemistry equipment and supplies Condensed Matter Physics biology.organism_classification Molecular biology Atomic and Molecular Physics and Optics Cell biology medicine.anatomical_structure Modeling and Simulation embryonic structures Blastoderm |
| Zdroj: | Journal of Nanoparticle Research. 13:6895-6906 |
| ISSN: | 1572-896X 1388-0764 |
| DOI: | 10.1007/s11051-011-0597-3 |
| Popis: | Due to their unique fluorescent characteristics, quantum dots (QDs) have been successfully applied in the fields of biotechnology and medicine, but there is very limited information regarding their biodistribution and chronic toxicity in vivo. In this article, the biological behavior and toxic effects of mercaptoacetic acid-CdSe/ZnS QDs (MAA-QDs) in developing zebrafish embryos were investigated by in vivo tests. The MAA-QDs were introduced into zebrafish through microinjection at early stage. The results showed that the MAA-QDs at certain concentrations influenced the survival of zebrafish embryos, but treated embryos without developmental defects were also observed. MAA-QDs injected into the cytoplasm at the one-cell stage were allocated to progeny blastoderm cells during proliferation and almost never entered the yolk. The formation of notochord and primordial germ cells with normal morphologies was detected in the treated embryos by whole-mount in situ hybridization. Furthermore, traces of the element cadmium were mainly discovered in the tissue of liver and kidney of 3-month-old-treated zebrafish by quantitative assessment with inductively coupled plasma mass spectrometry. Thus, we hypothesized that low concentration MAA-QDs have chronic toxicities when they were delivered into zebrafish organs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|