Phenotypes of B-Lymphocytes as a Predictor of Steroid Response in Idiopathic Nephrotic Syndrome
| Autor: | Hoda A. Fathey, Lobna A. El-Korashi, Doaa Mohammed Youssef, Faika Arab, Fatma Ibrahim |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
education.field_of_study biology medicine.diagnostic_test business.industry medicine.medical_treatment Population Venous blood medicine.disease Gastroenterology CD19 Steroid Flow cytometry Pathogenesis medicine.anatomical_structure Internal medicine medicine biology.protein education business Nephrotic syndrome B cell |
| Zdroj: | GEGET. 15:28-35 |
| ISSN: | 2636-3666 |
| DOI: | 10.21608/geget.2021.49509.1016 |
| Popis: | Introduction: The pathogenesis of Idiopathic nephrotic syndrome (INS) is not clear, where the role of B-cells is not yet illustrated. Aim of the study: To evaluate B- cells subsets as a predictor of steroid therapy sensitivity among INS at initial onset. Methods: 45 children with idiopathic nephrotic syndrome were recruited in a follow- up study, patients were divided into steroid-sensitive nephrotic syndrome (SSNS) and steroid-resistant nephrotic syndrome (SRNS) groups based on their sensitivities to steroids. Venous blood samples were drawn from all children for assessment of memory B- cell population before and after one year of steroid therapy. Results: We found statistically significant relation between steroid response and total memory CD19+ CD27+B cell at disease onset where it was higher in SSNS group. Our results showed The best cut-off of total CD19+ B cells in predicting steroid response was ≥32.7 with an area under curve (AUC) of 0.445, a sensitivity of 43.9%, a specificity of 50%, a positive predictive value (PPV) of 90%, a negative predictive value (NPV) of 8% and an accuracy of 44.4% (p>0.05) We also found that The best cut-off of CD19+ CD 27+ total memory B cells in predicting steroid response was ≥32 with an AUC of 0.817, a sensitivity of 78%, a specificity of 75%, a PPV of 97%, a NPV of 25% and an accuracy of 77.8% (p < 0.05). Conclusion: CD19+ CD 27+ total memory B cells could help in predicting steroid response among INS patients with a cut-off of total CD 19+B cells above 32.7 and PPV of 90% , a cut-off above 32 in CD19+ CD +27 total memory cells and PPV 97%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|