Ceftriaxone sodium loaded onto polymer-lipid hybrid nanoparticles enhances antibacterial effect on gram-negative and gram-positive bacteria: Effects of lipid - polymer ratio on particles size, characteristics, in vitro drug release and antibacterial drug efficacy
| Autor: | Mohammad Karimi, Nafiseh Farhadian, Arash Rigi Hossein abadi, Samaneh Porozan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
biology
Ceftriaxone Sodium medicine.drug_class Gram-positive bacteria Antibiotics Pharmaceutical Science 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification 030226 pharmacology & pharmacy Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Polymer ratio Glycerol monostearate medicine 0210 nano-technology Bacteria Nuclear chemistry Enterococcus faecium |
| Zdroj: | Journal of Drug Delivery Science and Technology. 63:102457 |
| ISSN: | 1773-2247 |
| DOI: | 10.1016/j.jddst.2021.102457 |
| Popis: | This study investigates the ability of hybrid polymer-lipid nanoparticles (PLN) containing ceftriaxone sodium (CTX) drug to enhance the mortality rate of Escherichia coli (E. coli), as gram-negative, and Enterococcus faecium (E-faecium), as gram-positive bacteria. PLN samples were prepared using a self-assembly method with different lipid-polymer mass ratios (L/P) ranging from 0.2 to 0.7. PLNs consisted of chitosan as a biocompatible polymer, glycerol monostearate as a lipid and Tween 80 as a stabilizer. Characterization results based on FESEM, FTIR, DSC and XRD analyses confirmed the successful formation of a polymer-lipid matrix. In vitro drug release showed that L/P ratio could significantly control the rate and amount of drug released from nanoparticles (NPs), including burst release and sustained release, in which PLN with an L/P ratio of 0.5 had desirable sustained drug release. This sample had a positive effect on the mortality rate of E. Coli bacteria compared to other PLN samples. In addition, compared to other conventional forms of antibiotics, the resistance of E. faecium bacteria in patients with cellulitis was reduced to half by applying these NPs. In conclusion, results confirmed that hybrid PLN provides an appropriate nanostructure for the delivery of hydrophilic antibiotics such as CTX with lower bacteria resistance. |
| Databáze: | OpenAIRE |
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