Cloning and Characterization of Partial Promoter of HMGCoA Reductase from Andrographis paniculata (Burm.f.) Wall.ex Nees — A Tropical Medicinal Plant

Autor:	M. G. Purushothama, K. Seetha, R. V. Omkumar, N. S. Banerjee
Rok vydání:	2005
Předmět:	Genetics Cloning biology TATA box Promoter Plant Science Reductase Molecular cloning biology.organism_classification Molecular biology Primer walking Agronomy and Crop Science Gene Andrographis paniculata Biotechnology
Zdroj:	Journal of Plant Biochemistry and Biotechnology. 14:41-44
ISSN:	0974-1275 0971-7811
DOI:	10.1007/bf03263223
Popis:	Hydroxymethylglutaryl-CoA (HMGCoA) reductase, a key enzyme of the mevalonate pathway is known to play a key role in the biosynthesis of terpenoids. Here we describe, the cloning and characterization of hmgr1 promoter from Andrographis paniculata. A 710 bp partial promoter fragment including a putative TATA box, 5’ UTR and 5’ coding sequence of the gene was cloned by amplifying the upstream sequences using PCR with appropriate primers. Analysis of the promoter sequence against PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative light dependent regulatory cis-elements in the sequence along with TATA and CAAT boxes. RT-PCR detected GUS transcripts in transgenic plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7a51c52984ce9fa634326981d64c63e7 https://doi.org/10.1007/bf03263223 Zobrazit plný text záznamu Full text from SpringerLink