Preparation of Porous Protein‐Based Hydogel for Highly Sensitive Protein Chips
| Autor: | Toshifumi Shiroya, Hiroyuki Tanaka, Hisao Takeuchi, Tatsushi Isojima, Haruma Kawaguchi, Minako Hanasaki |
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| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Materials science Polymers and Plastics biology Organic Chemistry technology industry and agriculture Substrate (chemistry) Nanoparticle Polymer Conjugated system Condensed Matter Physics Chemical engineering chemistry Polymer chemistry Materials Chemistry Protein microarray biology.protein Polymer blend Self-assembly Protein A |
| Zdroj: | Macromolecular Symposia. 266:81-84 |
| ISSN: | 1521-3900 1022-1360 |
| DOI: | 10.1002/masy.200850615 |
| Popis: | Proteins are important tools for high-throughput analysis of biological events. We have developed a novel method to prepare a protein-based hydrogel, that is, a Three-Dimensional Nano-structured Protein Hydrogel (3-D NPH), which is composed of protein and polymer nano-particles. The 3-D NPH could be easily prepared by dispensing a protein and polymer mixture on a substrate. Surprisingly, gold particles conjugated with protein A diffused into the 3-D NPH which was made of mouse IgG through the pores. We have shown that the protein chips made with our 3-D NPH method has tremendously improved sensitivity in detecting protein-protein interactions compared with that of direct protein immobilization methods. |
| Databáze: | OpenAIRE |
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