| Autor: | D. Schmitt, Y. Neveux, Pradel D, Reano A, J. Bergier, E. Gentilhomme |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Skin repair
Basement membrane integumentary system Health Toxicology and Mutagenesis Sulfur mustard Cell Biology Biology Toxicology Molecular biology Fibronectin chemistry.chemical_compound medicine.anatomical_structure chemistry Toxicity Immunology biology.protein medicine Skin equivalent Fibroblast Filaggrin |
| Zdroj: | Cell Biology and Toxicology. 14:1-11 |
| ISSN: | 0742-2091 |
| DOI: | 10.1023/a:1007491703271 |
| Popis: | Skin intoxication by bis(β-chloroethyl)sulfide (BCES; sulfur mustard) induces cutaneous lesions similar to thermal burns, characterized by slowness of skin healing. We have developed an in vitro model of skin equivalent to investigate mechanisms involved in this delay. Direct intoxication of dermal equivalent produced dose- and time-dependent cytotoxicity. A decrease of macroscopic retraction of collagen gels was observed, parallel to the toxic concentration with, at histological level, absence of collagen fiber reorganization. Fibroblast synthesis of fibronectin was also inhibited by intoxication, as demonstrated at an immunobiochemical and immunohistochemical level. These dermal alterations were correlated with secondary modifications of epithelial maturation of nonintoxicated normal human keratinocytes. Cellular adhesion was perturbed, as visualized by a delay in expression and reorganization of basement membrane components, laminen, collagen IV, and fibronectin. Epidermal terminal differentiation was also affected, as shown by the absence of profilaggrin/filaggrin biosynthesis. |
| Databáze: | OpenAIRE |
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