Popis: |
A study was designed to access the phytoremediation potential of microbial-assisted Chromolaena odorata of lead (Pb). The pots experiment was carried out in a Factoria experiment in a Completely Randomized Design, with nine treatments and three replications. Viable seeds of Chromoleana odorata were planted in 10kg of soil placed in each plastic pot having 10ppm, 20ppm, and 30ppm of Pb, respectively. Seedlings were inoculated with either Pseudomonas aeruginosa or Bacillus subtilis. The study was carried out for 12 weeks under natural conditions. Physiochemical properties of the soil were determined using standard methods. The results revealed that the microbes (Pseudomonas aeruginosa and Bacillus subtilis) used showed the potential for remediating lead. However, C. odorata inoculated with Bacillus subtilis remediated lead (Pb) was significantly higher than that inoculated with Pseudomonas aeruginosa at 10 weeks after planting. The levels of Pb in the roots and shoot of C. odorata after 12 weeks (B10, 0.22) showed that a more bioavailable pool of Pb was translocated from the root to leaves and stem in the Bacillus subtilis assisted C. odorata compared with other treatments. The results suggested that C. odorata has phytoextraction ability and could restore soil polluted with Pb. Bacillus subtilis assisted C. odorata to extract Pb via phytoaccumulation in shoot while Pseudomonas aeruginosa assisted C. odorata in removing Pb via phytostabilization. It also suggested that plant growth-promoting bacteria helped the uptake of lead from the soil. |