Comparison of the early innate immune responses of Vibrio parahaemolyticus and Vibrio cholerae in human intestinal epithelial cells (P1237)
| Autor: | Candace Winstead, Evan Buck, Jad El Adaimi, Thomas Moores, Tyler Slocum, Misty Moyle |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 190:56.1-56.1 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.190.supp.56.1 |
| Popis: | Diarrheal disease is one of the leading causes of death in low-income countries. Two pathogens responsible for these diseases are Vibrio parahaemolyticus and Vibrio cholerae. Although from the same genus, they have distinct virulence factors and induce different host responses. This study evaluates the early immune response of human intestinal epithelial cells (HT-29) to Vibrio pathogens by comparing changes in gene expression induced by the bacteria using quantitative PCR (qPCR) arrays. 84 different genes in an anti-bacterial inflammatory response were compared in cells stimulated in triplicate for two hours with V. parahaemolyticus and V. cholerae. Overall, V. cholerae upregulated many more genes (24 total) than V. parahaemolyticus (11 total). Both species induced expression of the pattern recognition receptor TLR1 and the chemokine CCL5 as well as unexpectedly increasing expression of several putative antiviral mediators including DDX58, IFNGR1 and MHC I. Genes upregulated by only V. parahaemolyticus include IL-8, IRAK-1, MAPK-8, and TLR-5, whereas V. cholerae upregulated NFKB1A and ICAM-1. Interestingly, many more genes were downregulated by V.parahaemolyticus (31 total) compared to V. cholerae (15 total), and several genes downregulated by V. parahaemolyticus (CCR4, TLR4, TLR6 and IL13) were upregulated by V. cholerae. This comparative expression approach with related pathogens may allow patterns to emerge that will guide future experiments. |
| Databáze: | OpenAIRE |
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