Production of Macroglobulins in Vitro and a Study of Their Cellular Origin

Autor:	Norman S. Cooper, Dorothea Zucker-Franklin, Edward C. Franklin
Rok vydání:	1962
Předmět:	Pathology medicine.medical_specialty Lymphoblast Immunology Macroglobulinemia Cell Biology Hematology Buffy coat Biology Biochemistry Macroglobulin Tissue culture medicine.anatomical_structure medicine Lymph Bone marrow Lymph node
Zdroj:	Blood. 20:56-64
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v20.1.56.56
Popis:	Lymph nodes of three patients with macroglobulinemia of Waldenström were studied in tissue culture and shown to synthesize 19S γ-globulin in vitro. Lymph node imprints, bone marrow, and buffy coat smears of the same patients consisted almost entirely of lymphocytes. When these were stained with fluorescein-conjugated antiserum to macroglobulin, large and medium-sized lymphocytes and lymphoblasts rather than mature lymphocytes or plasma cells were shown to contain the protein. It is suggested that 19S γ-globulin may also be synthesized by cells belonging to the lymphoid series under normal circumstances.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7a2211d0bc1a994307a6b67a68c7d45d https://doi.org/10.1182/blood.v20.1.56.56 Zobrazit plný text záznamu