Portable multi-spectral lens-less microscope with wavelength-self-calibrating imaging sensor
| Autor: | Yinxu Bian, Xu Liu, Anwar Hussian, Diyi Liu, Zhifeng Zhang, Cuifang Kuang, Haifeng Li, Qiulan Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Microscope
business.industry Mechanical Engineering Holography 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials law.invention 010309 optics Lens (optics) Optics law 0103 physical sciences Microscopy Imaging technology Electrical and Electronic Engineering Image sensor 0210 nano-technology business Phase retrieval Digital holography |
| Zdroj: | Optics and Lasers in Engineering. 111:25-33 |
| ISSN: | 0143-8166 |
| DOI: | 10.1016/j.optlaseng.2018.07.009 |
| Popis: | Multi-spectral imaging technology is helpful to obtain better contrast and more details of images at different wavelengths compared with monochromatic imaging or conventional RGB imaging technologies. And in resource-limited areas, microscopes with features of low-cost and portability are extremely attractive. In this manuscript, we demonstrate that cost-effective lens-less microscopy can be combined with the multi-spectral imaging technology, which can be alternative to conventional approaches and applied in biology, chemistry, environmental science. As a proof of this concept, we present a novel portable multi-spectral lens-less microscope (MSLLM). An outstanding highlight of our setup is its wavelength-self-calibrating imaging sensor (WSCIS), which can provide computationally optimized wavelength value (COWV) for LED illumination in real time. For each illumination, 5 holograms of the sample at 5 different heights are captured. Through the COWVs and phase retrieval algorithms, 7 multi-spectral images (FOV: 4.8 mm*4.6 mm, resolution: ∼1 µm) can be reconstructed. We believe that our MSLLM can be widely applied to multi-spectral microscopy in low-cost required scenes. |
| Databáze: | OpenAIRE |
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