Evaluation of the Detection Methods used for Investigation of Listeria and Listeria monocytogenes

Autor:	Abrahão Wm, de Francisco Tg, de Souza Vr, Bernardi G, Pontarolo R, Benetti Tm
Rok vydání:	2015
Předmět:	medicine.diagnostic_test Listeria monocytogenes biology business.industry Immunoassay medicine Listeria Food science Gold standard (test) business medicine.disease_cause biology.organism_classification Microbiology
Zdroj:	Journal of Food and Nutritional Disorders.
ISSN:	2324-9323
Popis:	Evaluation of the Detection Methods used for Investigation of Listeria and Listeria monocytogenes This study investigated the occurrence of Listeria spp. in samples of fresh cheese and ricotta comparing the results obtained using the conventional methodology (ISO 11290-1) and automated enzyme immunoassay systems (mini- VIDAS®LIS, mini-VIDAS®LMO2, mini-VIDAS®LDUO-LIS and mini-VIDAS®LDUO-LMO). The conventional method was used as gold standard for the methods comparisons. The sensitivities for the identification of Listeria by mini-VIDAS®LIS and mini-VIDAS®LDUO-LIS were 73.3% and 64.7%, respectively. For L. monocytogenes identification the sensitivities were 83.3% (mini-VIDAS®LMO2) and 62.5% (mini- VIDAS®LDUO-LMO). The specificity parameter obtained by the mini-VIDAS® systems ensured the detection of the target microorganism.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::79f683eb9fc31214deb2cebefa7c15a9 https://doi.org/10.4172/2324-9323.1000184 Zobrazit plný text záznamu