Distribution of Xanthomonas hortorum pv. carotae Populations in Naturally Infested Carrot Seed Lots
| Autor: | Jeremiah K. S. Dung, Jeness C. Scott |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine education.field_of_study Pathogen detection Population Plant Science 030108 mycology & parasitology Biology biology.organism_classification medicine.disease_cause 01 natural sciences Crop 03 medical and health sciences Horticulture Xanthomonas hortorum Infestation medicine Bacterial blight education Agronomy and Crop Science 010606 plant biology & botany Daucus carota |
| Zdroj: | Plant Disease. 104:2144-2148 |
| ISSN: | 1943-7692 0191-2917 |
| DOI: | 10.1094/pdis-12-19-2674-re |
| Popis: | Bacterial blight of carrot (Daucus carota subsp. sativus), caused by the plant-pathogenic bacterium Xanthomonas hortorum pv. carotae, is a common seedborne disease of carrot wherever the crop is grown. Carrot seed lots were evaluated to determine the variability and distribution of populations of X. hortorum pv. carotae among individual carrot seeds. Twenty-four carrot seed lots harvested between 2014 and 2016 were subjected to a bulk seed wash dilution-plate assay to obtain mean X. hortorum pv. carotae levels. Mean infestation levels resulting from the bulk seed wash assays among the 24 seed lots ranged from 1.2 × 107 and 9.6 × 108 CFU/g seed and averaged 3.6 × 108 CFU/g seed. Individual seeds from the same 24 lots were also tested with a scaled-down wash assay of individual seeds. Among the 1,380 seeds that were individually assayed, 475 X. hortorum pv. carotae-positive seeds were detected (34.4%). Rates of X. hortorum pv. carotae detection on individual seed in seed lots ranged from 0% (not detected) to 97.9%, and the mean and median X. hortorum pv. carotae population on an individual seed was 8.3 × 104 and 6.3 × 101 CFU/seed, respectively. Among individual seeds, X. hortorum pv. carotae populations ranged from 2 (the limit of detection of the assay) to 3.6 × 107 CFU/seed. CFU data for 23 of the 24 seed lots were nonnormal and the Log-Logistic (3P) distribution best described populations of X. hortorum pv. carotae recovered from individual carrot seeds. The influence and impact of nonnormal distributions of X. hortorum pv. carotae in commercial carrot seed lots on seed health tests, seedborne transmission, and bacterial blight epidemiology requires further study. |
| Databáze: | OpenAIRE |
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