MICROEMULSION ELECTROKINETIC CHROMATOGRAPHY OF β-CAROTENE AND ASTAXANTHIN
| Autor: | Leena Suntornsuk, Brompoj Prutthiwanasan, Khin Thida Nyunt Nyunt |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Chromatography medicine.medical_treatment Clinical Biochemistry Carotene Pharmaceutical Science Biochemistry Analytical Chemistry chemistry.chemical_compound Electrokinetic phenomena Capillary length chemistry Pulmonary surfactant Astaxanthin medicine Microemulsion Sodium acetate Carotenoid |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 36:671-686 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1080/10826076.2012.673206 |
| Popis: | This research aimed to separate β-carotene and astaxanthin by microemulsion electrokinetic chromatography (MEEKC) using oil in water (o/w) and water in oil (w/o) microemulsions. Acid o/w MEEKC (pH 2.5) was inappropriate due to the instability of the carotenoids under acid conditions, while basic o/w MEEKC (pH 9.2) gave poor separation with low sensitivity. Alternatively, w/o MEEKC was developed by varying concentrations and types of oil, surfactant, additional oil, and capillary length. The optimum condition was in w/o MEEKC containing 9% w/w SDS, 80% w/w 1-butanol, 11% w/w 70 mM sodium acetate buffer (pH 8), using a temperature of 25°C, a separating voltage of −30 kV, the capillary effective length of 23.5 cm, and a detection wavelength at 475 nm. w/o MEEKC provided the baseline separation of carotenoids with a resolution of 4.9 in 8 min. Linear ranges of both carotenoids were 20–120 µg/mL with the regression equations of y = 1.16x + 12.59 (r2 = 0.996) and y = 1.01x + 2.26 (r2 = 0.997) for astaxanthin an... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |