| Autor: |
Marc Montalà-Flaquer, Clara F. López-León, Daniel Tornero, Tanguy Fardet, Pascal Monceau, Samuel Bottani, Jordi Soriano |
| Rok vydání: |
2022 |
| Popis: |
SUMMARYNeuronal cultures are a prominent experimental tool to understand complex functional organization in neuronal assemblies. However, neurons grown on flat surfaces exhibit a strongly coherent bursting behavior with limited functionality. To approach the functional richness of naturally formed neuronal circuits, here we studied neuronal networks grown on polydimethylsiloxane (PDMS) topographical patterns shaped as either parallel tracks or square valleys. We followed the evolution of spontaneous activity in these cultures along 20 daysin vitrousing fluorescence calcium imaging. The networks were characterized by rich spatiotemporal activity patterns that comprised from small regions of the culture to its whole extent. Effective connectivity analysis revealed the emergence of spatially compact functional modules that were associated to both the underpinned topographical features and predominant spatiotemporal activity fronts. Our results show the capacity of spatial constraints to mold activity and functional organization, bringing new opportunities to comprehend the structure-function relationship in living neuronal circuits. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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