Microsatellite marker development for the federally listed Prunus geniculata (Rosaceae)1

Autor:	Douglas E. Soltis, Charlotte C. Germain-Aubrey, Matthew A. Gitzendanner, Pamela S. Soltis
Rok vydání:	2011
Předmět:	Florida scrub Rosaceae Plant Science Biology biology.organism_classification Phylogeography Prunus Botany Genetic variation Genetics Microsatellite Prunus geniculata Allele Ecology Evolution Behavior and Systematics
Zdroj:	American Journal of Botany. 98:e58-e60
ISSN:	1537-2197 0002-9122
DOI:	10.3732/ajb.1000435
Popis:	 Premise of the study: Prunus geniculata is a federally listed species endemic to the central Florida scrub. To investigate levels and geographic partitioning of genetic variation in this species, we developed microsatellite markers for P. geniculata and its sister species, P. maritima .  Methods and Results: Following a protocol optimized in our laboratory, we designed 36 primer pairs, of which 8 amplifi ed across both species and showed informative polymorphism. Product size varied from 163 to 370 bp, and the number of alleles ranged from 5 to 27. H e across all populations ranged from 0.604 to 0.9155.  Conclusions: Eight loci were useful at the population level and will be used for conservation genetic and phylogeographic studies of Prunus geniculata and P. maritima . Furthermore, from fi ve to all eight of these loci can also be amplifi ed in several other North American Prunus species.
Databáze:	OpenAIRE
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