| Autor: |
R H Wasserman, C S Fullmer |
| Rok vydání: |
1979 |
| Předmět: |
|
| Zdroj: |
Journal of Biological Chemistry. 254:7208-7212 |
| ISSN: |
0021-9258 |
| Popis: |
Peptide mapping of underivatized tryptic digests of bovine and chick intestinal calcium-binding proteins has been accomplished by high performance liquid chromatography (HPLC). High precision analysis of nanomolar quantities of peptides were achieved in less than 1 h (recycle time). Peak resolution and definition are superior compared to conventional techniques and recoveries of both small (4-residue) hydrophilic and large (30-residue) hydrophobic peptides are excellent. The total amino acid composition of the bovine intestinal calcium-binding protein has been accounted for on the basis of two tryptic maps of 20 microgram of protein each. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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