Effective Substrate Loading for Saccharification of Corn Cob and Concurrent Production of Lignocellulolytic Enzymes by Fusarium oxysporum and Sporothrix carnis
Autor: | Oluwatosin I. Oni, Folasade M. Olajuyigbe, Cornelius O. Fatokun |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
Marketing Pharmacology chemistry.chemical_classification Organizational Behavior and Human Resource Management biology Chemistry 020209 energy Strategy and Management Pharmaceutical Science Substrate (chemistry) Sporothrix 02 engineering and technology biology.organism_classification 01 natural sciences Hydrolysis Enzyme 010608 biotechnology Drug Discovery Fusarium oxysporum 0202 electrical engineering electronic engineering information engineering Food science |
Zdroj: | Current Biotechnology. 8:109-115 |
ISSN: | 2211-5501 |
DOI: | 10.2174/2211550108666191008154658 |
Popis: | Background: One of the critical challenges of cost-effective bioethanol production from lignocellulosic biomass is the decreasing yield of reducing sugars caused by increasing substrate loading. Hence, it is crucial to determine the best substrate concentration for efficient saccharification of lignocellulosic wastes. Objective: This paper reports the saccharification of corn cob by two lignocellulolytic fungi (Fusarium oxysporum and Sporothrix carnis) and concurrent production of lignocellulolytic enzymes at varying substrate concentrations. Methods: F. oxysporum and S. carnis were cultivated on corn cob based media at 30°C and 160 rpm for 144 h. The lignocellulosic composition of corn cob was determined. Saccharification of varying concentrations of substrate was determined by evaluating the release of reducing sugar while the production of cellulase and xylanase was monitored. Results: Cellulose, hemicellulose and lignin contents of corn cob were 37.8±1.56%, 42.2±1.68% and 12.7±1.23%, respectively. Yields of reducing sugar by F. oxysporum and S. carnis were 5.03 µmol/mL and 6.16 µmol/mL; and 6.26 µmol/mL and 6.58 μmol/mL at 10.0 and 25.0% substrate concentration, respectively. The production of cellulase and xylanase was exponential as corn cob concentration increased from 0.5% to 10.0% yielding 586.93 U/mL and 1559.18 U/mL from F. oxysporum, with 590.7 U/mL and 1573.95 U/mL from S. carnis, respectively. Conclusion: The study shows that the most efficient saccharification of corn cob by F. oxysporum and S. carnis was achieved at 10.0% substrate concentration. This suggests that two separate saccharification processes at this concentration will result in higher yields of enzyme and reducing sugars than a single process involving higher concentration. |
Databáze: | OpenAIRE |
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