| Popis: |
The activity and molecular organization of NAD + kinase have been studied throughout the life cycle of the silkworm, Bombyx mori . The apparent molecular weights of the enzyme forms revealed by 3–20% polyacrylamide gel electrophoresis were determined to be; I, 138,000; II, 152,000; III, 182,000 and IV, 205,000 daltons. The pattern and relative percentage composition of the molecular forms, as well as the specific activity of NAD + kinase, were shown to undergo changes in the transition from one developmental phase to another. Form I of the NAD + kinase is present only at the end of embryogenesis, form II is characteristic of actively growing larvae, form III is present at all developmental stages, except the end of embryogenesis, while form IV appears at the stages when development is provided by endogenic energy resources. |
