The yeast lysyl-tRNA synthetase gene. Evidence for general amino acid control of its expression and domain structure of the encoded protein

Autor:	Marc Mirande, Jean-Pierre Waller
Rok vydání:	1988
Předmět:	chemistry.chemical_classification TATA box Structural gene C4A Nucleic acid sequence Protein primary structure Cell Biology Biology SYT1 Biochemistry Molecular biology Amino acid chemistry HSPA2 Molecular Biology
Zdroj:	Journal of Biological Chemistry. 263:18443-18451
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(19)81378-9
Popis:	The nucleotide sequence of a 3.6-kilobase pair DNA fragment containing the structural gene for yeast cytoplasmic lysyl-tRNA synthetase (KRS1) and its flanking regions was determined. The encoded protein of 67,881 kDa displays a cluster of 11 lysines within a 29-amino acid residue segment at its amino-terminal extremity. Evidence is presented that this segment is responsible for the affinity displayed by the native enzyme toward polyanionic carriers. The transcription initiation sites of the KRS1 gene were determined. Upstream from the TATA box, putative control elements corresponding to the concensus sequences for the RPG box and the general amino acid control system were identified. Evidence for transcriptional induction of the KRS1 gene via the general amino acid control system is presented.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::798f6313fefd8d13392a5ca0744e6431 https://doi.org/10.1016/s0021-9258(19)81378-9 Zobrazit plný text záznamu