Microbiological Assay for Saponin in Alfalfa Products
| Autor: | Livingston Al, Whitehand Lc, Kohler Go |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
chemistry.chemical_classification
Ethanol Aqueous solution biology fungi Microbiological assay Trichoderma viride Saponin food and beverages General Chemistry Fungus biology.organism_classification complex mixtures carbohydrates (lipids) chemistry.chemical_compound chemistry parasitic diseases Botany Bioassay Potato dextrose agar Food science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 60:957-960 |
| ISSN: | 0004-5756 |
| DOI: | 10.1093/jaoac/60.4.957 |
| Popis: | A bioassay is described for determining medicagenin-type saponin in dried alfalfa, leaf protein concentrates, and alfalfa sprouts. Samples are extracted by refluxing 2½ hr with 50% ethanol, ethanol is evaporated, and aliquots of an aqueous solution are added to potato dextrose agar (PDA) and assayed for saponin by using the fungus Trichoderma viride. The growth of the fungus on PDA is compared with a standard saponin, and saponin levels are calculated by means of a slope ratio analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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