Effect of (−)-Epicatechin on Poorly Crystalline and Amorphous Precipitate. The Role of Green Tea Compound in the Formation of Infectious Urinary Stones

Autor:	Jolanta Prywer, Ewa Mielniczek-Brzóska
Rok vydání:	2019
Předmět:	Aqueous solution biology Nucleation General Chemistry Condensed Matter Physics biology.organism_classification law.invention Amorphous solid chemistry.chemical_compound Ammonia chemistry Optical microscope Struvite law General Materials Science Crystallization Bacteria Nuclear chemistry
Zdroj:	Crystal Growth & Design. 20:148-156
ISSN:	1528-7505 1528-7483
DOI:	10.1021/acs.cgd.9b00936
Popis:	Infectious urinary stones induced to grow by urease-producing bacteria consist of aggregates of bacteria, highly crystalline struvite, and poorly crystalline and amorphous precipitate (PCaAP). The paper describes an experimental study on influence of (-)-epicatechin, a green tea component, on the nucleation and growth of PCaAP. The crystallization process of PCaAP was performed in artificial urine, and the activity of bacteria was simulated by the addition of aqueous ammonia solution. The formation of struvite was avoided by using Mg-free artificial urine. Optical microscopy methods and spectrophotometric measurements both confirm that (-)-epicatechin promotes the nucleation and growth of PCaAP. This means that PCaAP nucleation is shifted towards a lower pH compared to the control test. The results of the experiments are explained based on the theoretical analysis of the chemical species formed in artificial urine. This chemical speciation analysis shows that the experimentally observed effects are due to...
Databáze:	OpenAIRE
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