Isolation of spilanthol from Acmella oleracea based on Green Chemistry and evaluation of its in vitro anti-inflammatory activity
| Autor: | Fernando A. Cabral, Veronica Santana de Freitas Blanco, Ícaro Augusto Maccari Zelioli, Barbara Michalak, Adriana da Silva Santos de Oliveira, Marili V.N. Rodrigues, Antonio G. Ferreira, Anna K. Kiss, Rodney Alexandre Ferreira Rodrigues, Vera Lúcia Garcia |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Green chemistry biology Human neutrophil Traditional medicine medicine.drug_class General Chemical Engineering Spilanthol Condensed Matter Physics biology.organism_classification 01 natural sciences Anti-inflammatory In vitro 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Column chromatography chemistry Active compound medicine Physical and Theoretical Chemistry Acmella oleracea |
| Zdroj: | The Journal of Supercritical Fluids. 140:372-379 |
| ISSN: | 0896-8446 |
| Popis: | Acmella oleracea (jambu) is a South American plant whose pharmacological properties include antimalarial, anesthetic, and anti-inflammatory activities. However, the compounds contributing to the anti-inflammatory effect have received little attention. In this study, the active compound spilanthol was isolated using a Green Chemistry approach and presented significant anti-inflammatory activity in a lipopolysaccharide-activated human neutrophil model. First, the aerial parts of jambu were extracted and fractionated using supercritical carbon dioxide. The spilanthol-enriched fractions obtained were then subjected to further purification by flash chromatography, yielding spilanthol with 97% purity. Use of enzyme-linked immunosorbent assay (ELISA) revealed a reduction in the release of Interleukin-8 and Tumor Necrosis Factor - alpha by leukocytes exposed to spilanthol. In conclusion, this new approach enabled spilanthol to be obtained at high purity, in a fairly rapid procedure, while the results of the in vitro anti-inflammatory activity study indicated that the compound could be a promising new therapeutic agent. |
| Databáze: | OpenAIRE |
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