High-level soluble expression of isopenicillin N synthase isozymes in E. coli
| Autor: | John D. Sutherland, Jonathan Michael Blackburn, Jack E. Baldwin, M.C. Wright |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
biology Organic Chemistry Isopenicillin N synthase medicine.disease_cause biology.organism_classification Penicillium chrysogenum Biochemistry Isozyme chemistry.chemical_compound Enzyme chemistry Biosynthesis Aspergillus nidulans Drug Discovery Gene expression biology.protein medicine Escherichia coli |
| Zdroj: | Tetrahedron. 47:5991-6002 |
| ISSN: | 0040-4020 |
| Popis: | The Isopenicillin N Synthase genes from Cephalosporium acremonium, Penicillium chrysogenum and Aspergillus nidulans have been expressed at high levels in Escherichia coli under control of the trc or lac promoters. The enzymes are produced in soluble form in contrast to previously reported expression in insoluble form under control of the λP L promoter. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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