Changes of abundance and diversity of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in three nitrifying bioreactors with different ammonia concentrations
| Autor: | Guixia Wu, Jingfeng Gao, Ting Li, Kai-Ling Pan, Xiao-Yan Fan |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
biology 030106 microbiology Ocean Engineering biology.organism_classification Pollution Microbiology 03 medical and health sciences Nitrososphaera Ammonia chemistry.chemical_compound Wastewater chemistry Nitrosomonas europaea Environmental chemistry Bioreactor Sewage treatment Bacteria Water Science and Technology Archaea |
| Zdroj: | Desalination and Water Treatment. 57:21463-21475 |
| ISSN: | 1944-3986 1944-3994 |
| DOI: | 10.1080/19443994.2015.1123196 |
| Popis: | Different from ammonia-oxidizing bacteria (AOB), the influence of ammonia levels on ammonia-oxidizing archaea (AOA) in wastewater treatment systems was poorly understood. In this study, sludge contained AOA and AOB was enriched in three sequencing batch reactors (named as R1, R2, and R3) under different ammonia levels (14, 56, and 140 mg/L) using polymerase chain reaction (PCR), cloning, sequencing, and quantitative real-time PCR (qPCR) to evaluate the effect of ammonia levels on the diversity, abundance, and contribution of AOA and AOB to ammonia oxidation. Cloning results showed that there was only one dominant AOA species (Nitrososphaera cluster) in the reactors during 140-d operation. However, AOB communities varied significantly among the reactors. After 140-d enrichment, Nitrosomonas ureae cluster, the dominant AOB cluster in seed sludge, was also dominant in R1 and R2, while Nitrosomonas europaea cluster was enriched and dominated in R3. Diversity of AOB was higher than AOA under the three ... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|