Laminin Receptors in Differentiated Thyroid Tumors: Restricted Expression of the 67-Kilodalton Laminin Receptor in Follicular Carcinoma Cells1
| Autor: | Nunzia Montuori, Mario Vitale, Stefano De Riu, Mark E. Sobel, Gianfranco Fenzi, Guido Rossi, Frank Müller |
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| Rok vydání: | 1999 |
| Předmět: |
Basement membrane
endocrine system medicine.medical_specialty Goiter Adenoma biology Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry Thyroid Integrin medicine.disease Biochemistry Endocrinology medicine.anatomical_structure Laminin Internal medicine Gene expression Follicular phase medicine biology.protein |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 84:2086-2092 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.84.6.5721 |
| Popis: | The expression of integrin laminin receptors was investigated in normal thyroid primary cultures; immortalized normal thyroid cells (TAD-2); papillary (NPA), follicular (WRO), and anaplastic (ARO) thyroid tumor cell lines; seven thyroid tumors (four papillary and three follicular carcinomas); and normal thyroid glands. The expression of α1β1, α2β1,α 3β1, α6β1, andα 6β4 was found in all tumor specimens and in tumor cell lines, whereas normal thyroid cells and TAD-2 cells lacked the expression of α6β4. Despite the presence of several integrin laminin receptors, adhesion of TAD-2, NPA, and ARO cells to immobilized laminin-1 was poor, whereas WRO cells and follicular carcinoma-derived cells displayed a strong adhesion. Indeed, WRO and follicular carcinoma-derived cells showed expression of a nonintegrin laminin receptor, the 67-kDa high affinity laminin receptor (67LR). TAD-2, NPA, and ARO cells as well as nodular goiter, toxic adenoma, follicular adenoma, and papillary carcinoma-derived cells did not express the 67LR. Adhesion of WRO and follicular carcinoma-derived cells to laminin-1 was specifically inhibited by a recombinant polypeptide containing laminin-binding domains of 67LR, demonstrating that this receptor confers to follicular carcinoma cells attachment capacity to laminin. Moreover, tissue specimens from follicular carcinomas expressed the 67LR, whereas follicular adenomas and normal thyroid tissues were negative. In thyroid tumors, integrin receptors, although abundant, participate weakly in adhesion to laminin. The expression in follicular carcinoma cells of a functional, high affinity 67LR together with nonfunctional integrin LM receptors could be responsible for the tendency of follicular carcinoma cells to metastasize by mediating stable contacts with basal membranes. |
| Databáze: | OpenAIRE |
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