Radiolabeled Complex of Antimicrobial Peptides UBI29-41 and UBI18-35 Labeled with 99mTc for Differential Diagnosis of Bone Infection of the Limbs
| Autor: | A. S. Rogov, Vladimir V. Ivanov, M. V. Belousov, V. D. Zavadovskaya, A. Ya. Lushchyk, V. D. Udodov, A. V. Yantsevich, N. M. Shevtsova, V. S. Skuridin, Alexandra G. Pershina, M. A. Zorkaltsev, M. S. Larkina, A. N. Baikov, E. S. Stasyuk |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Osteomyelitis Antimicrobial peptides Inflammation General Medicine medicine.disease digestive system General Biochemistry Genetics and Molecular Biology Bone Infection 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Biochemistry medicine Chelation Differential diagnosis medicine.symptom Bifunctional Technetium-99m 030217 neurology & neurosurgery |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 170:415-419 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-021-05078-7 |
| Popis: | We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI18-35, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide-1-yl 6-(bis(pyridin-2-ylmethyl)amino)hexanoate was used. The obtained complexes 99mТс-DPAH-UBI29-41 and 99mТс-DPAH-UBI18-35 had radiolabeling yield >80% and radiochemical purity >96%. Accumulation of the complexes in the focus of bacterial inflammation in bone structures and the absence of this complex in the site of aseptic inflammation was confirmed in a rat model of traumatic osteomyelitis by single-photon emission computed tomography. |
| Databáze: | OpenAIRE |
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