Engineered Minichromosomes in Plants
| Autor: | Changzeng Zhao, Rick E. Masonbrink, James A. Birchler, Robert T. Gaeta, Lakshminarasimhan Krishnaswamy |
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| Rok vydání: | 2010 |
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| Zdroj: | Critical Reviews in Plant Sciences. 29:135-147 |
| ISSN: | 1549-7836 0735-2689 |
| Popis: | Engineered minichromosomes provide the ability to target transgenes to a defined insertion position for predictable expression on an independent chromosome. This technology promises to provide a means to add many genes to a synthetic chromosome in sequential manner. An additional advantage is that the multiple transgenes will not be inserted into the normal chromosomes and thus will not exhibit linkage drag when converging the transgenes to different germplasm nor will they be mutagenic. Telomere truncation coupled with the introduction of site-specific recombination cassettes has proven to be an easy method to produce minichromosomes. Telomere truncation results from the transformation of plasmids carrying a block of telomere repeats at one end. Minichromosomes consisting of little more than a centromere have been produced for B chromosomes of maize. Such small chromosomes have been studied for their meiotic behavior, which differs from normal sized chromosomes in that homologue pairing is rare or nonexi... |
| Databáze: | OpenAIRE |
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