A novel fluorescent protein chromophore analogue to simultaneously probe lysosome viscosity and β-amyloid fibrils
| Autor: | Jinsheng Liu, Han Sun, Gaurab Roy, Jin-wu Yan, Hua-xiang Leng, Lei Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
Chemistry
Metals and Alloys 02 engineering and technology Chromophore 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Fibril 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Biomarker (cell) medicine.anatomical_structure β amyloid Lysosome Materials Chemistry medicine Biophysics Diagnostic biomarker Fluorescent protein Electrical and Electronic Engineering 0210 nano-technology Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 305:127509 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2019.127509 |
| Popis: | Alzheimer's disease (AD) is an incurable neurodegenerative disease. Better diagnosis of AD is extremely important for therapeutic interventions. The amyloid cascade hypothesis and its revised version identify insoluble β-amyloid deposition as a good diagnostic biomarker for AD. We hypothesize that lysosomal viscosity may also act as an auxiliary biomarker for AD based on literature research. Herein, we have successfully developed a FP chromophore analogue C25 as a dual-functional fluorescent probe which can respond effectively to the Aβ fibrils and enhanced lysosomal viscosity with distinguishable turn-on fluorescence signals around 570 nm and 600 nm, respectively. The probe C25 can also monitor the Aβ degradation in the lysosomes. The probe C25 can serve as a promising tool for developing early diagnosis and innovative therapeutics of AD. |
| Databáze: | OpenAIRE |
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